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Black Sacrifice (Kuro no Ikenie - 黒の生贄) is the third episode of the anime adaptation of the Bible Black game and the third episode of the Bible Black: La Noche de Walpurgis series.

It was first released in Japan on the 21st of December, 2001 and in the United States on the 19th of November, 2002 under the name Second Sacrament in double feature containing both this episode and the fourth episode Black Caress. This episode was later rereleased in Japan as a special remastered version (see below).

As Walpurgis Night approaches, the evil Academy nurse Reika Kitami reveals her history and true nature when torturing and raping art professor Hiroko Takashiro. With Taki Minase, Kaori Saeki and her witchcraft club friends Jun and Maki firmly under her control, she arranges the rape of Minase's girlfriend Rika Shiraki and kidnap of his childhood friend Kurumi Imari to use as a vessel for her soul.

In the apartment building used by Saeki's coven, Academy art professor Hiroko Takashiro is being water tortured at Reika Kitami's command. A rubber hose is lodged in her mouth and water is filling up her insides. She desperately gasps for air when Kaori Saeki pulls it out. The girl hands Taki Minase a large plastic syringe to fill her up even more.

Takashiro screams and struggles when Minase uses the syringe to push water inside her body. Kitami sadistically revels in the torturous spectacle, saying her suffering speaks to her like a work of art.
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